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Thymosin beta-4

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by hatchet » August 31, 2007 at 12:42 pm #56442

I have been researching trying to figure out which proteins, amino acids, peptides, etc., to add to my gentle wounding regimen, in addition to LiCl/DMSO. I have several ideas but I’m getting overloaded with info…

I ran across thymosin beta-4 (), and it sounds good, but so far I have only found it commercially available in Jan Mari products for skin care, which also contain TGF beta-1, which has been shown to play a role in hair loss. So I’m thinking that wouldn’t be a good combo.

I just want the thymosin beta-4 without the tgf beta-1. I know it’s been discussed before but now it is available at least. It is being used in clinical trials/studies to regrow tissue in wound healing, repair organs, etc.

Here is some info from another site:

Thymosin beta4 increases hair growth by activation of hair follicle stem cells.

Philp D, Nguyen M, Scheremeta B, St-Surin S, Villa AM, Orgel A, Kleinman HK, Elkin M.
Cell Biology Section, NIDCR, National Institutes of Health, Bethesda, Maryland 20892, USA.

“Thymosin beta4, a 43-amino acid polypeptide that is an important mediator of cell migration and differentiation, also promotes angiogenesis and wound healing. Here, we report that thymosin beta4 stimulates hair growth in normal rats and mice. A specific subset of hair follicular keratinocytes in mouse skin expresses thymosin beta4 in a highly coordinated manner during the hair growth cycle. These keratinocytes originate in the hair follicle bulge region, a niche for skin stem cells. Rat vibrissa follicle clonogenic keratinocytes, closely related, if not identical, to the bulge-residing stem cells, were isolated and their migration and differentiation increased in the presence of nanomolar concentrations of thymosin beta4. Expression and secretion of the extracellular matrix-degrading enzyme matrix metalloproteinase-2 were increased by thymosin beta4. Thus, thymosin beta4 accelerates hair growth, in part, due to its effect on critical events in the active phase of the hair follicle cycle, including promoting the migration of stem cells and their immediate progeny to the base of the follicle, differentiation, and extracellular matrix remodeling”.

Also, Thymuskin products have thymosin, too, but is the beta-4 form? I’m not sure. But they claim it basically does the same thing regarding stem cells, etc.

“ThymuSkin® contains thymosin, which is applied topically and absorbed through the scalp and into the blood. Several studies have found that thymosin promotes hair growth in normal and aged rodents. A 2004 study examined the effects of thymosin on hair follicle stem cell growth. ‘Taken together, our results suggest that thymosin beta4 exerts a profound hair-promoting effect through a combined action on several critical events in hair follicle growth such as stem cell migration, ECM-degrading enzyme production, and differentiation,’ the study authors write.”

I know people have tried Thymuskin, but I wonder how it would work in conjunction with the gentle wounding for possible regeneration.

I am also considering this product just to see if all the glycoproteins, amino acids, etc., would help.

Notice the snail secretion stuff…it is interesting. I found another product using snail secretions called CAS also. All the talk about stem cells, regeneration, etc., has me interested.

Or maybe this one:

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hatchet

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by angstman » August 31, 2007 at 3:37 pm #56449

sounds like good stuff. im surprised i havent seen or heard of it before on here.

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by hatchet » August 31, 2007 at 8:14 pm #56451

Here’s more info on thymosin from Le Magazine, published in 2002:

Thymosin beta 4 and Skin Repair

by Carmia Borek, Ph.D.

The promise of repairing sun parched aging skin is alluring, especially if damage control may be attained by applying a substance that is abundant in our body. Thymosin beta 4 (Tb4), a molecule that accelerates wound healing in animals and cultured cells, “may be valuable in repairing skin damage caused by sun or even by the wear and tear of aging?” This hopeful message of Tb4′s potential to restore damaged human skin was voiced at the 5th International Symposium on Aging Skin, in California (May 2001), by Dr. Allan Goldstein, Chairman of the Biochemistry Department at George Washington University and founder of RegeneRX Biopharmaceuticals. RegeneRX is carrying out preclinical research on Tb4 as a wound healer, in collaboration with scientists at the National Institutes of Health.

Skin is the largest organ of the body, which makes up 16% of total body weight. It is also the largest organ that provides immune protection and plays a role in inflammation. Composed of specialized epithelial and connective tissue cells, skin is our major interface with the environment, a shield from the outside world and a means of interacting with it. As such, the skin is subjected to insults and injuries: burns from the sun’s ultraviolet radiation that elicit inflammatory reactions, damage from environmental pollutants and wear and tear that comes with aging.

There are several layers in the skin; the outer epidermis and beneath it the dermis and the subcutaneous layer. Cells in the epidermis include keratinocytes, its major cell type, that move continuously from the lower basal layer where they are formed by cell division. Other cells in the epidermis are the melanocytes that synthesize pigment and transfer it to the keratinocytes, giving our skin its color, and a wide variety of immune cells that maintain immune surveillance and secrete substances called cytokines, like interleukin 1 and 2, which are active in inflammation. The dermis contains connective tissue, mainly collagen, blood vessels, various types of immune white cells and fibroblasts.

The structure that provides the cell with form is the cytoskeleton, whose protein actin, a housekeeping molecule in cells, comprises 10% of the cell protein. Actin is essential for cell division, cell movement, phagocytosis (engulfing foreign bodies in immunoprotection) and differentiation.

Cells on the surface of the skin are constantly being replaced by regeneration from below. The repair of a wound is a scaling up of this normal process, with additional complex interactions among cells, formation of new blood vessels, collagen, more extensive cell division and cell migration, as well as strict control of inflammatory cells and the cytokines they release to resolve the inflammation.

Skin damage and aging are induced to a large extent by free radicals from the sun and environmental pollutants and from oxidants produced during infection and inflammation. Lipid peroxidation of membranes and increased inflammatory substances, such as thromboxanes and leukotriens, add insult to injury. While skin damage accumulates with age, repair processes slow down. Thus, any boost by a molecule that would reduce free radicals and accelerate molecular events in healing has the potential to hasten skin repair. Tb4 has such healing qualities.

The nature of Tb4

Thymosin beta 4 is a small 43 amino acid protein (a peptide) that was originally identified in calf thymus, an organ that is central in the development of immunity. Tb4 was later found in all cells except red blood cells. It is highest in blood platelets that are the first to enter injured areas, in wound healing. Tb4 is also detected outside cells, in blood plasma and in wound and blister fluids.

Its unique potential as a healing substance lies in that it interacts with cellular actin and regulates its activity. T

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hatchet

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by hatchet » August 31, 2007 at 8:19 pm #56452

I know, this is about rats, and probably been posted before, but here it is. Sounds very interesting if used along with gentle wounding:

Thymosin beta4 increases hair growth by activation of hair follicle stem cells.

Philp D, Nguyen M, Scheremeta B, St-Surin S, Villa AM, Orgel A, Kleinman HK, Elkin M
Cell Biology Section, NIDCR, National Institutes of Health, Bethesda, Maryland 20892, USA.

Thymosin beta4, a 43-amino acid polypeptide that is an important mediator of cell migration and differentiation, also promotes angiogenesis and wound healing. Here, we report that thymosin beta4 stimulates hair growth in normal rats and mice. A specific subset of hair follicular keratinocytes in mouse skin expresses thymosin beta4 in a highly coordinated manner during the hair growth cycle. These keratinocytes originate in the hair follicle bulge region, a niche for skin stem cells. Rat vibrissa follicle clonogenic keratinocytes, closely related, if not identical, to the bulge-residing stem cells, were isolated and their migration and differentiation increased in the presence of nanomolar concentrations of thymosin beta4. Expression and secretion of the extracellular matrix [?]-degrading enzyme matrix metalloproteinase-2 [?] were increased by thymosin beta4. Thus, thymosin beta4 accelerates hair growth, in part, due to its effect on critical events in the active phase of the hair follicle cycle, including promoting the migration of stem cells and their immediate progeny to the base of the follicle, differentiation, and extracellular matrix [?] remodeling.

FASEB J. (2004)
PMID: 14657002

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hatchet

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by hatchet » August 31, 2007 at 8:20 pm #56453

And here is a link to the complete study:

I think Jacob mentioned this somewhere in one of the threads about wounding/lithium, etc., now that I remember, but I didn’t know what he was talking about. I just now made the connection….

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hatchet

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by hatchet » September 11, 2007 at 8:18 pm #57136

Just bumping this up. I think thymosin beta 4 might be the ideal peptide to combine with the gentle wounding/LiCl/DMSO that I’m already doing. Anybody other than me interested in it? I have found a couple of potential sources. It was talked about a few years ago, but seems to have died down. A company in China, Lee Pharma, was developing a topical but put it on hold. It is used already in an anti-aging cream from Jan Mari (but it also contains TGF b1), but I found some sources for the pure stuff. I may try it. Thoughts?

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hatchet

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by BobTheBuilder » September 11, 2007 at 8:27 pm #57138

i am, but wont be back until monday.

I have contact my friend that is in the pharmaceutical company to see if he can get his hands on the acell stuff.

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by hatchet » September 11, 2007 at 8:30 pm #57139

Sounds good, Bob. I would love to know what Acell’s main ingredients are….

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by whovian » September 11, 2007 at 11:33 pm #57146

Found this:

‘Thymosin-beta-4 (TB4) is a small polypeptide that inhibits the migration of macrophages, and stimulates the secretion of hypothalamic luteinizing hormone-releasing hormone. It has also been implicated in wound healing, (see Malinda et al. (1999) J. Invest. Dermatol. 113:364-368). The molecule is ubiquitous; it had been found in all tissues and cell lines analyzed, but is found in highest concentrations in spleen, thymus, lung, and peritoneal macrophages. The polypeptide sequence of TB4 may be found in U.S. Pat. No. 4,297,276, Goldstein et al. The corresponding genetic sequence is described by Gondo et al. (1987) J. Immunol. 139 (11), 3840-3848, Genbank accession number M17733. Preferably human recombinant TB4 is used, which is commercially available, e.g. from Advanced ChemTech, Inc. (Louisville, Ky.), at a specific activity of 5 mg/1000 U. In the compositions of the present invention, the TB4 is used at a concentration of at least about 1 ng/ml, usually at least about 10 ng/ml, more usually at least about 100 ng/ml, and not more than about 10 µg/ml, more usually not more than about 1 µg, and may be used at a concentration of about 0.1 to 0.5 µg/ml.’

Advanced ChemTech, Louisville, KY

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by JHarsh80 » September 12, 2007 at 1:25 am #57148

Hatchet,

How long have you been wounding, and what are your results so far?

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by Steve the Searcher » September 12, 2007 at 5:11 am #57152

Sorry Hatchet but Thymosin Beta 4 is old hat.It has been discussed here and in other forums over the past few years.

It never came out as a hair loss treatment possibly because of the potential for tumours which are a danger in all wound healing substances if they cannot be safely regulated.

I was interested in this but as it never took off despite the common knowledge there must have been some problems encountered in the Laboratory stage testing.

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by cpio_ » September 12, 2007 at 7:11 am #57159

I use Thymuskin topical hair treatment.
It is very expensive, but I use it for
three reasons:

It is a very effecient anti inflammatory.
It contains coffeine that reduces DHT in
the scalp. And lastly, it is supposed to
have a permanent effect on hair loss, since the tiny thymus particles penetrates
into the follicle, and over time accumulates so DHT can no longer attach to the follicle. That way hair loss
should begin to diminish “on its own”
over time.

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by Jacob » September 12, 2007 at 7:34 am #57161

Steve..the potential for tumors is not the reason. I’ve yet to read that those working on TB4 for hair loss have decided not to go ahead with it. Obviously they’d be watching things carefully and looking for the correct dosages/# of applications/etc.

TB4 is still going strong on it’s wound healing ability btw.

The problem with Thymuskin is it doesn’t contain much TB4 and then there’s the stability issue(maybe). If you’re looking for a TB4 source that is.

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by hatchet » September 12, 2007 at 9:52 am #57177

Lee Pharma just has it listed as uncertain as far as production of their topical but not as dropped. I suspect it may be more of a patent issue than anything else. And I know they are still using it in wound healing trials, so I don’t think they would be doing that if it were already proven to cause tumors. Also, if that were the case, Jan Mari wouldn’t be putting it in their anti-wrinkle creams.

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by Jacob » September 12, 2007 at 12:12 pm #57194

But they bought the patent rights to it. I seem to recall when they were answering emails the latest was they were working on stability issues.

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