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| Title: Potassium channel openers stimulate DNA synthesis in mouse
epidermal keratinocyte and whole hair follicle cultures. |
| Title Abbreviation: Skin Pharmacol |
Date of Pub: 1993 |
| Author: Harmon CS; Lutz D; Ducote J; |
| Issue/Part/Supplement: 3 |
Volume Issue: 6 |
Pagination: 170-8 |
| MESH Headings: Animal; Benzopyrans (PD); Cells, Cultured; Diazoxide
(PD); DNA (*BI); Guanidines (PD); Hair (DE/*ME); Keratinocytes (DE/*ME); Kinetics;
Mice; Minoxidil (PD); Potassium Channels (DE/*PH); Pyrroles (PD); Vibrissae (PH); -RN-; |
| Journal Title Code: AOA |
Publication Type: JOURNAL ARTICLE |
| Date of Entry: 940204N |
Entry Month: 9404 |
| Country: SWITZERLAND |
Index Priority: 2 |
| Language: Eng |
Unique Identifier: 94099973 |
| Unique Identifier: 94099973 |
ISSN: 1011-0283 |
| Abstract: We have conducted studies using primary mouse epidermal
keratinocyte and whole hair follicle cultures to investigate the mechanism of the
hypertrichotic activity of potassium channel openers. In a time course study, the extent
of stimulation of epidermal keratinocyte DNA synthesis by minoxidil increased as the rate
of DNA synthesis in control cultures declined. Minoxidil stimulation of DNA synthesis in
7-day cultures required prolonged (> 1 day) exposure to the agent. Pinacidil and
diazoxide also stimulated DNA synthesis in mouse epidermal keratinocyte cultures. In
addition, minoxidil, pinacidil, diazoxide, and cromakalim stimulated DNA synthesis
in whole-organ cultures of mouse hair follicles. These results suggest that
potassium channel openers retard the loss of proliferative activity of differentiating
keratinocytes and support the hypothesis that these agents stimulate hair growth
through a direct effect on hair follicles. |
| Abstract By: Author |
| Address: Roche Dermatologics, Hoffmann-La Roche Inc., Nutley, NJ
07110. |
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