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Hair Loss Study Abstract: Finasteride inhibits 5 alpha-reductase activity in human dermal fibroblasts: prediction of its therapeutic application in androgen-related skin diseases.
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Title
Finasteride inhibits 5 alpha-reductase activity in human dermal fibroblasts: prediction
of its therapeutic application in androgen-related skin diseases.
Author
Nguyen QH; Chen T; Wang X; Chen Y; Chien P
Address
Institute of Chemical Biology, University of San Francisco, CA 94117-1080, USA.
Source
Int J Dermatol, 34: 10, 1995 Oct, 720-5
Abstract
BACKGROUND. The potential role of finasteride in treating androgen related skin
disorders was investigated. METHODS. Pooled human dermal fibroblasts were used to assess
the effect of finasteride on the 5 alpha-reductase activity in skin tissue. Vmax and Km
were estimated in the presence of 0, 10, and 200 nM finasteride. RESULTS. Vmax values
remain constant near 1.20 pmol/mg protein/h in the presence of increasing concentrations
of finasteride; however, apparent Km increases from 0.27 nM at 0 nM finasteride to 0.31 nM
and 0.44 nM at 10 nM and 200 nM finasteride, respectively. This suggests that finasteride
competes with testosterone and has a high affinity for same binding site of the 5
alpha-reductase enzyme. Apparent Ki was estimated at 282 nM, indicating that a high
concentration of finasteride is required to significantly suppress the enzyme activity.
CONCLUSIONS. This study confirms that finasteride inhibits the conversion of testosterone
to dihydrotestosterone in human reticular dermal fibroblasts. Finasteride may have
therapeutic potential in treating skin disorders influenced by the action of
dihydrotestosterone.
Language of Publication
English
Unique Identifier
96122064
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