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Hair Loss Study Abstract: Isolation and maintenance of the human pilosebaceous duct: 13-cis retinoic acid acts directly on the duct in vitro.
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Title
Isolation and maintenance of the human pilosebaceous duct: 13-cis retinoic acid acts
directly on the duct in vitro.
Author
Guy R, Ridden C, Barth J, Kealey T
Address
Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital,
U.K.
Source
Br J Dermatol, 128: 3, 1993 Mar, 242-8
Abstract
The human pilosebaceous duct was isolated and maintained for 7 days in defined medium,
and defined medium supplemented with 1 microM 13-cis retinoic acid. Freshly isolated ducts
retained their in vivo morphology, showing a stratified squamous keratinizing epithelium.
On maintenance there was a loss of basic duct architecture, and a significant reduction in
the rate of [methyl-3H] thymidine uptake. The addition of 1 microM 13-cis retinoic acid
resulted in an improved duct architecture and caused a further significant reduction in
the rate of [methyl-3H] thymidine uptake. [Methyl-3H] thymidine autoradiography showed
that freshly isolated ducts maintained their in situ pattern of cell division. It was
difficult to discern the region of cell division in ducts maintained for 7 days, but the
degree of graining reflected the measured rates of [methyl-3H] thymidine uptake into PCA
precipitable material. The pattern of keratin synthesis of the freshly isolated duct was
similar to patterns previously described for the duct in situ. This study reports the
successful isolation and maintenance of the human pilosebaceous duct, and demonstrates
that 13-cis retinoic acid acts directly at the level of the duct.
Language of Publication
English
Unique Identifier
93229346
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