|
|
|
Hair Loss Study Abstract: Testosterone metabolism in primary cultures of human prostate epithelial cells and fibroblasts.
|
Title
Testosterone metabolism in primary cultures of human prostate epithelial cells and
fibroblasts.
Author
Délos S; Carsol JL; Ghazarossian E; Raynaud JP; Martin PM
Address
Laboratoire de Cancérologie Expérimentale, Faculté de Médecine Secteur Nord,
Marseille, France.
Source
J Steroid Biochem Mol Biol, 55: 3-4, 1995 Dec, 375-83
Abstract
We compare testosterone (T) metabolism in primary cultures of epithelial cells and
fibroblasts separated from benign prostate hypertrophy (BPH) and prostate cancer tissues.
In all cultures, androstenedione (delta 4) formed by oxidation of T by 17
beta-hydroxysteroid dehydrogenase (17 beta-HSD) represented 80% of the metabolites
recovered. The amounts of 5 alpha-dihydrotestosterone (DHT), formed by reduction of T by 5
alpha-reductase (5 alpha-R), were small: 5 and 2% (BPH) and 8 and 15% (adenocarcinoma) for
epithelial cells and fibroblasts, respectively. Northern blot analysis of total RNA from
epithelial cells (BPH or adenocarcinoma) attributed the reductive activity to the 5
alpha-reductase type 1 isozyme and oxidative activity to the 17 beta-HSD type 2. In cancer
fibroblasts, only little 17 beta-HSD type 2 mRNA was detected. The 5 alpha-reductase
inhibitors, 4-MA (17 beta-(N,N-diethyl)carbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one)
and finasteride, inhibited DHT formation with a preferential action of 4-MA on epithelial
cells (BPH or adenocarcinoma) and of finasteride on fibroblasts from adenocarcinoma.
Neither inhibitor acted on delta 4 formation. On the other hand, the lipido-sterol extract
of Serenoa repens (LSESr, Permixon) inhibited the formation of all the T metabolites
studied [IC50 S = 40 and 200 micrograms/ml (BPH) and 90 and 70 micrograms/ml
(adenocarcinoma) in epithelial cells and fibroblasts, respectively]. These results have
important therapeutic implications when selecting appropriate treatment options for BPH.
Language of Publication
English
Unique Identifier
96132689
content2 |
|
